June 25, 2007
Researchers have successfully used a virus to combat bacteria that cause middle ear infections in mice. Lysin, the protein derived from the virus, could help stave off the 8 million cases of middle ear infections that strike children each year.
In PLoS Pathogens, Rockefeller University’s Vincent A. Fischetti and colleagues at St. Jude show that treatment with lysin for middle ear infections in mice can be effective. The new technology may prevent children with the flu from developing secondary ear infections and would be an attractive alternative to traditional antibiotics, to which bacteria are rapidly becoming resistant.
The bacteria that cause middle ear infections, Streptococcus pneumoniae, aren’t transmitted at school. They already reside on the mucosal membranes in the nose, waiting for their chance to strike. When a child catches the flu, or another virus that causes an upper respiratory infection, the bacteria seize the opportunity and migrate to the middle ear, causing a secondary infection. The new treatment would kill the bacteria before it had a chance to move.
"These bacteria take advantage of a viral infection by striking when our resistance is lowered," says Fischetti, head of Rockefeller’s Laboratory of Bacterial Pathogenesis and Immunology. "You should start taking an antibiotic the moment you come down with the viral infection to prevent the secondary infection, but physicians are reluctant to do this for fear of increasing resistance to antibiotics. So we’re really in a catch-22 situation."
Fischetti’s lab has done extensive research on lysins, which are proteins derived from viruses that normally infect bacteria. After a virus has infected the bacteria and replicated, it uses lysins to punch holes in the bacteria’s cell wall, killing the bacteria, in order to escape. Fischetti’s lab has studied many different lysins and found that they work even from outside the bacterial cell as well as from the inside. In addition, unlike antibiotics, which kill many of the body’s beneficial bacteria along with the disease-causing ones they target, lysins are highly specific. Each lysin will only kill a specific type of bacteria, leaving the body’s normal flora untouched.
"We knew from our previous experiments that if you treat an animal infected or colonized with pneumococci or streptococci with these lysins, you could cure or decolonize them," says Fischetti. "But I was looking for a way to see if lysins would work for secondary infections too, and John McCullers had a very nice model system."
The two researchers collaborated – Fischetti sent McCullers, a physician at St. Jude Children’s Hospital Department of Infectious Diseases, the lysin, and McCullers tested his mice. He colonized his mice with S. pneumoniae, treated some of them with the lysin and then gave them all influenza. Eighty percent of the mice that did not receive the lysin came down with middle ear infections, but none of the mice given the lysin did. The treatment was 100 percent effective.
"It is really a no-brainer experiment," says Fischetti. "If the bacteria aren’t there, they cannot cause the secondary infection."
"Secondary bacterial infections cause much of the sickness and about 25 percent of all deaths during flu season," says McCullers. "Eliminating these secondary infections could dramatically reduce sickness and death rates."